In vitro propagation of the genus Actinidia Lindl. representatives

The features of regeneration of the genus Actinidia Lindl. representatives at the main stages of clonal micropropagation were studied. The best results for viability of primary explants were achieved during sterilization in 2% Fundazole solution with an exposure of 10 min, followed by 30 s in 70% ethanol and 5-7 min in 7% calcium hypochlorite with 1-2 drops of Tween-20. The determining value of species characteristics in the realization of morphogenetic potential was established. The cultivars of A. kolomikta were characterized by a lower multiplication coefficient compared to A. arguta and A. polygama. For the first time, the differences between the various forms (male, female, and bisexual) were revealed by the regeneration ability. A comparative analysis of various forms of Actinidia species showed that the female forms of A. kolomikta and A. arguta had higher multiplication coefficient than male and bisexual forms. It was shown that the use of the QL medium supplemented with 0.3-0.5 mg L^‑1 6-BAP was optimal for effective clonal micropropagation of the male forms; while, QL with 0.5-1.0 mg L^‑1 was effective for female and bisexual forms. The use of 0.5-1.0 mg L^‑1 IAA as an auxin at the rooting stage of the studied species contributed to a high root formation.