Evaluation of cannabinoid content in different cultivars of Cannabis indica L.

Our study on planting ten cultivars of Cannabis indica L. seeds (‘KKU-01’, ‘KKU-02’, ‘KKU-03’, ‘KKU-04’, ‘KKU-05’, ‘KKU-06’, ‘KKU-07’, ‘KKU-08’, ‘KKU-09’, and ‘KKU-10’) obtained from Khon Kaen Provincial Health Office gave various amounts of canabinoids content. The seeds of 8 males and 16 females were planted in a close system (the container cabin) by controlling soil material, light, water, and temperature. The cannabis plant flowers were collected after planting for 90 days to yield dry weight of plant of 45-88 g. The samples were subjected to analysis for two major cannabinoids, Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) by high performance liquid chromatography (HPLC). ‘KKU-01’, ‘KKU-09’, and ‘KKU-10’ yielded only THC at 7.55, 18.40, and 12.6% from dried weight, respectively. ‘KKU-03’ and ‘KKU-08’ produced mixtures of THC and CBD at 5.28 and 4.55% from dried weight, respectively. In addition, ‘KKU-05’ and ‘KKU-07’ yielded only CBD at 8.33 and 6.70% from dried weight, respectively.