Acclimatization of in vitro cultured pomegranate (Punica granatum L.)

K.B. Patil, J. Patil, K. Moharir, A.B. Patil, B.L. Jangale, B. Krishna
Pomegranate (Punica granatum L.) is an economically important fruit crop in arid and semi-arid tropics and subtropics because of its unique capacity to tolerate drought conditions. The pomegranate has medicinal, pharmaceutical and nutraceutical values. Pomegranate acreage has increased worldwide and also in India. In the tropics, pomegranate has been severely affected by bacterial blight and several other diseases. To establish disease free plantation in new areas, availability of quality planting material in large quantities is not possible through conventional means of propagation. The tissue culture laboratory of Jain Irrigation Systems Ltd. Jalgaon has developed an efficient in vitro propagation method for pomegranate. Being a delicate plant with a unique physiology, acclimatization of pomegranate plantlets is difficult and has a low success rate. Light, temperature, humidity and growing media play an important role in the acclimatization process. An effort was made to standardize a commercial acclimatization process for in vitro propagated pomegranate plants. A two-step (primary and secondary) acclimatization process was adopted with different varying conditions to develop an efficient procedure. In the primary acclimatization, the combination of 45% light and peat moss medium resulted in higher survival and better quality plantlets compared to other potting media and lights. For secondary acclimatization, the plants also grew best in a combination of 45% light (for the first 20 days) and peat moss medium. This treatment gave the best plant height, stem girth, number of leaves, leaf area, number of nodes, root volume, shoot weight and root weight. The studies provide valuable information on the choice of media and light intensity for acclimatization of tissue culture grown pomegranate.
Patil, K.B., Patil, J., Moharir, K., Patil, A.B., Jangale, B.L. and Krishna, B. (2023). Acclimatization of in vitro cultured pomegranate (Punica granatum L.). Acta Hortic. 1359, 95-104
DOI: 10.17660/ActaHortic.2023.1359.11
hardening, rooting media, light conditions, fruit

Acta Horticulturae