Reducing post-rooting dormancy in juneberry (Amelanchier alnifolia) using plant growth regulators and temperature treatments

N.K. Ardayfio, W. Dai, H. Hatterman-Valenti
A ten-week study was conducted to evaluate post-rooting dormancy of micropropagated juneberry. Temperatures of 6 and 25°C were main plots, while ‘Thiessen’ and ‘Northline’ cultivars (pre-rooted or non-pre-rooted), and nine plant growth regulator (PGR) treatments were sub-plots. Significant two-way and three-way interactions indicated the complexity of post-rooting dormancy and the difficulty when trying to overcome this dormancy. Pre-rooted plantlets treated with 750 mg L‑1 gibberellic acid (GA4+7), 100 mg L‑1 benzyladenine (BA) or 250 mg L‑1 GA4+7 + 100 mg L‑1 BA treatments recorded the greatest leaf number. ‘Thiessen’ pre-rooted plantlets grown at 25°C also had the greatest leaf number. Application of 250, 500 and 750 mg L‑1 GA4+7 for pre-rooted plantlets resulted in the tallest plants. Pre-rooted juneberry plantlets given 100, 200, 300 mg L‑1 BA and 250 mg L‑1 GA4+7 + 100 mg L‑1 BA had the greatest branching along with ‘Thiessen’ plantlets grown at 25°C. Pre-rooted ‘Thiessen’ plantlets had the most biomass as fresh weight and dry weight and produced the greatest root volume.
Ardayfio, N.K., Dai, W. and Hatterman-Valenti, H. (2023). Reducing post-rooting dormancy in juneberry (Amelanchier alnifolia) using plant growth regulators and temperature treatments. Acta Hortic. 1359, 43-56
DOI: 10.17660/ActaHortic.2023.1359.5
https://doi.org/10.17660/ActaHortic.2023.1359.5
benzyladenine, cytokinin, gibberellic acid, juneberry, saskatoon berry, tissue culture
English

Acta Horticulturae