Characterization of new endophytic bacterial strains isolated from brachiaria grass

Anh Le Phuong, Linh Vu Thi, Huong Bui Thu, Giang Van Nguyen
Brachiaria grass (Brachiaria brizantha) is a lengthy grass with deep roots and small rhizomes that grows 60-120 cm tall. This grass is cultivated in various parts of Vietnam and adapts well to various soil conditions. Brachiaria grass can be used for animals; therefore, forage cannot contain any chemical pesticide residues. Consequently, farmers prioritized using endophytic microorganisms derived from bioorganic fertilizers in brachiaria grass as a safe way to ensure quality and reduce food contamination in livestock. Strains were isolated according to the method described by Kumar et al. (2016). The experiment was carried out to select and evaluate some biological characteristics of endophytic bacterial strains from Brachiaria. From the different parts of the brachiaria grasses collected in Thai Nguyen and Ba Vi, 31 strains of endophytic bacteria were isolated, all 31 strains were able to produce IAA and degrade insoluble phosphate, and 24 strains were able to produce siderophores. The 6 potential strains selected are L-Br1-3, R-Br2-2, L-Br2-5, T-Br2-2, T-Br2-5, and R-Br2-4. The strains have inhibited 4 strains of plant pathogenic bacteria: Xanthomonas axonopodis, Ranstonia solanacearum, Clavibacter michiganensis, and Xanthomonas sp. The strain with the best activity, R-Br2-2, was selected for identification. The results of analysis and comparison of 16S rRNA nucleotide sequences of this strain in NCBI Genbank ( show that strain R-Br2-2 has a very close relationship (98.10%) with Bacillus pacificus MCCC 1A06182 should be named Bacillus pacificus R-Br2-2.
Le Phuong, Anh, Vu Thi, Linh, Bui Thu, Huong and Van Nguyen, Giang (2023). Characterization of new endophytic bacterial strains isolated from brachiaria grass. Acta Hortic. 1375, 259-268
DOI: 10.17660/ActaHortic.2023.1375.34
brachiaria grass, endophytic bacteria, Bacillus spp., biofertilizer, IAA

Acta Horticulturae