IN VITRO PROPAGATION OF P.S. B2 PEACH ROOTSTOCK.
Trials were carried out on the possibility of propagating P.S. B2 peach rootstock by in vitro culture. At the beginning of the trials a modified MS medium was used, adding BAP at 0.6, 1.2 and 2.4 mg/l. Apices, 10–12 mm in length, were taken from actively growing shoots developed on cuttings collected in winter and subsequently placed in a forcing chamber. During the initial 4 subcultures, explant growth was very poor: the apices developed only in length, with short internodes, large leaves and without axillary bud sprouting. Some of the explants were thus cultured on WPM medium to which 1.2 mg/l of BAP was added. Shoot growth was much improved; in fact, after the first 2 subcultures, shoot proliferation increased to a rate of 1:3.5, while on MS medium the explants still showed a lower growth rate. MS and WPM media were also tested for shoot elongation and rooting; best results (90% of rooting) were obtained by culturing the shoots on MS medium for the elongation phase and on WPM medium for rooting.
Morini, S. and Concetti, S. (1985). IN VITRO PROPAGATION OF P.S. B2 PEACH ROOTSTOCK.. Acta Hortic. 173, 205-210