INFLUENCE OF VARIOUS FACTORS ON PRODUCTION OF ALKALOIDS BY CLAVICEPS FUSIFORMIS ON PENNISETUM TYPHOIDES CALLUS CULTURES
Claviceps fusiformis Loveless and Pennisetum typhoides (Burm) Stapf and Hubb dual cultures were raised on modified Murashige and Skoog's medium to study the influence of various physical, nutritional and hormonal factors. Maximum alkaloid contents were recorded at 25°C. The light adversely influenced the alkaloid production which was maximum under continuous darkness followed by alternate light and darkness(8/16 hr), red (650 nm) blue (450 nm) and continuous fluorescent light(4000 lux). Incorporation of pyridoxine (0.5 ppm) promoted alkaloid production by dual cultures. Among different carbohydrates the inert sugar mannitol supported maximum alkaloid production which was followed by maltose, glucose and sucrose. Fructose inhibited the growth as well as alkaloid production. Apparently carbohydrate supply inhibited the alkaloid production. Among the different hormones incorporated separately the maximum alkaloid production was recorded on 2,4-D supplemented medium followed by NAA, Kinetin and GA3. IAA and BA showed comparatively less alkaloid contents as compared to the control.
Roy, S. and Kumar, A. (1986). INFLUENCE OF VARIOUS FACTORS ON PRODUCTION OF ALKALOIDS BY CLAVICEPS FUSIFORMIS ON PENNISETUM TYPHOIDES CALLUS CULTURES. Acta Hortic. 188, 91-98