J. Quinn, A. Whipkey, J. Simon, J. Janick
Borage seed contains several long-chain fatty acids with gamma-linolenic acid (GLA), a minor fatty acid of medicinal importance, as a major constituent. Seed development was complete about 20 days after pollination (DAP). Meiosis occurred 9 days before anthesis. The 4-cell stage, early heart-shape stage, cotyledon expansion, seed coat darkening, and abscission occurred at 1, 4, 5–16, 16–18, and 17–20 DAP, respectively. Developing borage embryos were analyzed at 2-day intervals, from 6 DAP until mature (18 DAP). Embryo accumulation of fatty acids (6.03 mg) and GLA (0.87 mg) accumulation reached a peak at 16 DAP. Somatic embryos and embryogenic-competent callus initiated from immature zygotic embryos produced GLA. There was a 3.6 fold increase in percent fatty acid and a 1.6 fold increase in percent GLA in cotyledonary somatic embryos relative to embryogenic callus.
Quinn, J., Whipkey, A., Simon, J. and Janick, J. (1987). EMBRYO DEVELOPMENT OF BORAGO OFFICINALIS. Acta Hortic. 208, 243-250
DOI: 10.17660/ActaHortic.1987.208.26

Acta Horticulturae