TISSUE CULTURE OF SMILAX ZEYLANICA L.
Callus cultures were established from stem explants of Smilax zeylanica L. on a modified basal medium of Murashige and Skoog (1962) supplemented with 5 mg/l Kn + 2 mg/l NAA + 1 mg/l 2,4-D + 2.5 mg/l IBA. Shoot buds develop after 8 weeks in the BM containing 5 mg/l Kn + 2.5 mg/l IBA. Combination of 1 mg/l BA + 0.05 mg/l 2,4-D was most suitable for shoot multiplication and development from differentiating calli. Rooting was induced when shoots were transferred to half strength MS inorganic salts supplemented with 2 mg/l IBA. The undifferentiated and differentiating calli contain higher content of diosgenin than the shoots.
Jha, S., Gupta, J. and Sen, S. (1987). TISSUE CULTURE OF SMILAX ZEYLANICA L.. Acta Hortic. 208, 273-279