FACTORS AFFECTING ESTABLISHMENT AND DEVELOPMENT OF MAGNOLIA HYBRIDS IN VITRO

Ilse E.G. Biedermann
Dormant axillary buds of various magnolia hybrids, 'Elizabeth', 'Yellow Bird', 'cultivar #149' and Magnolia stellata were cultured on Murashige-Skoog medium (MS), Anderson-Rhododendron medium (AR) and a medium containing lower concentrations of macronutrients than the other two. All media were supplemented with m-inositol, 500 mg/L; Jacquoit vitamins; benzyladenine, 5–15 mg/L; and napthaleneacetic acid, 1–5 mg/L. Cultures were maintained on a 16 hour photoperiod or total darkness at 21 C for 4 weeks. Cultures with 240 mg/L KNO3 concentration and carbohydrates of 1% at low light intensity had a greater survival rate and larger calli development. All hybrids did better on the medium containing low concentrations of macronutrients.
Biedermann, Ilse E.G. (1987). FACTORS AFFECTING ESTABLISHMENT AND DEVELOPMENT OF MAGNOLIA HYBRIDS IN VITRO. Acta Hortic. 212, 625-630
DOI: 10.17660/ActaHortic.1987.212.104
https://doi.org/10.17660/ActaHortic.1987.212.104

Acta Horticulturae