PROPAGATION OF THE FERN ADIANTUM CAPILLUS-VENERIS THROUGH TISSUE CULTURE OF THE CIRCINATE PART OF YOUNG LEAVES
The best morphogenic response was obtained with Gamborg B5 medium supplemented with IBA 0.5mg.1-1 and BA 0.01mg.1-1, 2% sucrose, at pH 5.5. After 8–10 weeks of culture, formed meristems were transferred to fresh medium without growth regulators (developing medium). Six weeks after, plantlets were transferred to the rooting medium (Gamborg B5 plus NAA 0.05mg. 1-1). Well developing plantlets, suitable for potting, were obtained after, at least, 1 month on this medium. A condition for plant growth is the soil composition and a relative high atmospheric humidity (about 100%).