CLONAL MASS PROPAGATION OF THE FERN CYRTOMIUM FALCATUM
Collected spores of Cyrtomium falcatum were sterilized according to Knauss method and germinated in an inorganic liquid medium as formulated by Dyer in 1969. The germination of the spores were observed within 7 to 9 days, and after 3 to 6 weeks both gametophytic and sporophytic stages were produced. Several gametophyte bearing sporophytic tissue were selected and divided in 4 to 6 sections. Each of these sections were cultured on Murashige and Skoog medium as revised by Leinsmaier and Skoog in 1965, and the effect of different substances on the regeneration of the complete plant was then analized. We tested sodium phosphate monobasic (255 mg/1), L-cystein (30 mg/1) and different concentrations of auxins: naphtaleneacetic acid (NAA), indole 3-butyric acid (IBA); and cytokinins: kinetin (K), 6-bencylaminopurine (BAP). Sucrose (20g/1) was added to all media. The induction of the formation of fast growing tissue with high regenerative characteristic was achieved either with K (2 mg/1) and ANA (0.1 mg/1) or with BAP (3 mg/1) and ANA (0.1 mg/1), but a better proliferation was observed in the medium with K and ANA. Differentiation of shoots and complete plantlets were obtained in a medium with lower salt concentration (Gamborg medium) and substituting IBA for ANA. For better elongation of the leaves gibberelic acid, GA (0.1 mg/1) was added. Between 30 to 50 plantlets per month were obtained in each flask after the regenerative tissue was established. The final amount depends on the size of the containers.
García, E. and Furelli, L. (1987). CLONAL MASS PROPAGATION OF THE FERN CYRTOMIUM FALCATUM. Acta Hortic. 212, 655-660