IN VITRO CULTURE OF SHOOT-TIP MERISTEMS IN SOME HIGHER PLANTS
In Solanum tuberosum, the aptitude for multiple shoots formation from the cultured meristems (meristem with 2 leaf primordia of approximally 0,5 mm in size) of different genotypes (4N : Bintje, Brettor, Victor, Ostara and 2N : 4 genotypes) were tested.
The best results were obtained with all the 4 diploid genotypes. The meristems were first cultured on the initiating medium (MS + Kinetin (0.1mg/1) + GA3 (0.1mg/1) + IAA (0.5mg/1). After 2 to 3 weeks these explants were transfered into the proliferating medium (MS + IAA 0.5mg/1) + BAP (1mg/1) + GA3 (0.5 mg/1) + Calcium panthothenate (2mg/1). In the above medium, multiple shoots were formed which were then placed on a rooting medium (with 0.01mg/1 IAA and only 1 % of sucrose in the MS medium). At most 60 % of the total number of meristems isolated developed into plants. Regenerated plantlets transfered to soil developed normally. No or very rare chromosomal abberation was found in these plants. (MS : MURASHIGE and SKOOG).