IN VITRO PROPAGATION OF HORSE-RADISH /COCHLEARIA ARMORACIA L./
Previous experiments proved that meristems of horse-radish taken from lateral buds of roots were very difficult to sterilize and poorly developed on growing media. To avoid this disadvantage, trials were carried out with leaf fragments in order to develop a method of rapid horse-radish propagation in vitro. Explants were triangular fragments of leaves with crossing of veins. The highest mean number of shoots /41.5/ from 1 leaf-fragment was obtained on medium LS containing IAA 0.8 + kin 4.0 mg per liter in comparison with 1.2 shoots obtained on the medium recommended. Frequently number of shoots on the best medium was over 50. On 2 media containing NAA few plantlets were already rooted in 4 weeks and ready for transplantation to the substrate. Repeating passages of individual shoot onto the same medium caused rapid outgrowth of numerous shoots. Shoots rooted very easily on all media containing auxines only. Plantlets survived in 96.5 % after transplantation to the external conditions. Also shoots planted directly into the potting substrate rooted easily in over 90 %.
Górecka, K. (1987). IN VITRO PROPAGATION OF HORSE-RADISH /COCHLEARIA ARMORACIA L./. Acta Hortic. 212, 671-674