MICROPROPAGATION OF TULIP: OPTIMISING SHOOT PRODUCTION FROM FLORAL STEM EXPLANTS
Considerable variation occurs in the number of shoots regenerated from floral stem explants of tulip cv. Merry Widow and this variation has been associated with factors such as explant's position within individual floral stems and the stage of bulb development. Explants excised and cultured in November (floral stems 14–15 mm in length) produced more shoots than those excised and cultured later. Proximity to the first node on the floral stem influenced the ability of explants to regenerate shoots. Explants cut into two or four pieces showed a higher potential than whole explants for shoot regeneration, especially when cultured in a liquid medium and filter paper bridge system. The effect of ethylene sources such as 1-aminocyclopropane1-carboxylic acid (ACC) and 2-chloroethylphosphonic acid (ethre1) is discussed briefly.
Taeb, A.G. and Alderson, P.G. (1987). MICROPROPAGATION OF TULIP: OPTIMISING SHOOT PRODUCTION FROM FLORAL STEM EXPLANTS. Acta Hortic. 212, 677-682