IN VITRO PROPAGATION AND REGENERATION OF PLANTS FROM CALLUSES OF BEGONIAX TUBERHYBRIDA
Tuberous begonias are traditionally propagated by seeds, except those belonging to the "multiflore" group which are propagated by cuttings. We describe a procedure for the in vitro micropropagation of Begonia x tuberhybrida and regeneration of plants from petiole calluses. The complete cycle, from in vitro induction of calluses to the flowering of regenerated potted plants, took 6 to 7 months (less than with the conventional methods). Mean multiplication rate was about 10 per month (with high values of 30) and the rate of successful establishment of plants in peat containers amounted 90%. The first flowers opened about 60 days after the plants were transferred to soil. Flowering was very homogeneous, and no variant phenotype was observed within 297 regenerated plants. After flowering, tuberisation was normal, the average diameter of the tubers being approximately 4 cm.
Viseur, J. and Lievens, C. (1987). IN VITRO PROPAGATION AND REGENERATION OF PLANTS FROM CALLUSES OF BEGONIAX TUBERHYBRIDA. Acta Hortic. 212, 705-710