SOMATIC EMBRYOGENESIS IN TISSUE CULTURE OF IRIS (IRIS PUMILA L.)
Mature seeds of a population designated P1/24 of an endemic iris species (Iris pumila L.) were cultured during one-month period in an appropriate medium. Embryos 1–2 mm long were isolated from germinated seeds and cultured on MS medium, containing 5% sucrose, vitamins and (in mg 1-1); 2,4-D 1.0, or IAA 1.0, kinetin 1.0, proline 250 and casein hydrolysate 200. Embryogenic callus was successfully induced only on medium containing 2,4-D. Somatic embryos were differentiated in the same medium during the second callus subculture. Further development of the embryos was achieved in liquid medium, and several plantlets were successfully transfered to normal conditions.
Plantlets regenerated from callus by somatic embryogenesis had the normal diploid chromosome number (2n = 32). Somatic embryogenesis did not involve qualitative and quantitative changes in isoenzyme pattern of several enzyme systems studies.
Radojevic, Lj., Sokic, O. and Tucic, B. (1987). SOMATIC EMBRYOGENESIS IN TISSUE CULTURE OF IRIS (IRIS PUMILA L.). Acta Hortic. 212, 719-724