ASPECTS AND PROSPECTS OF SOMATIC EMBRYOGENESIS IN MUSA, ABB, CV. BLUGGOE

N. Banerjee, J. Schoofs, S. Hollevoet, F. Dumortier, E. De Langhe
Thin meristematic layers, excised from proliferating shoot-tip cultures of Musa, ABB, cv. Bluggoe, produced callus when cultured on Murashige and Skoog's basal medium (MS) supplemented with 2,4-D (0.25 mg/1). On prolonged incubation, the callus developed numerous globular white proembryogenic masses all over the surface. Upon transferring to MS liquid medium devoid of 2,4-D and supplemented with other auxins and cytokinins, the proembryoids turned greenish and produced roots.

Although the apparent shoot apical region showed definite greening, no further leaf development was noticed. On the otherhand, proembryoids, obtained from MS liquid medium containing 2,4,5-T (1.25 mg/1) regenerated bipolar embryoids when transferred to fresh medium with NAA (0.02 mg/1) and BA (0.25 to 2.5 mg/1). The somatic embryos when compared histologically showed some resemblance with their zygotic counterparts. In both cases distinct bipolarity has been noticed where the shoot and the root primordia form a hook-like embryonal axis which is a characteristic feature of the members of Musaceae.

Banerjee, N., Schoofs, J., Hollevoet, S., Dumortier, F. and De Langhe, E. (1987). ASPECTS AND PROSPECTS OF SOMATIC EMBRYOGENESIS IN MUSA, ABB, CV. BLUGGOE. Acta Hortic. 212, 727-730
DOI: 10.17660/ActaHortic.1987.212.126
https://doi.org/10.17660/ActaHortic.1987.212.126

Acta Horticulturae