SOMATIC EMBRYOGENESIS AND PLANTLETS FORMATION FROM FLOWER BUDS CULTIVATED IN VITRO
Regeneration of plantlets from explanted strawberry flower-buds were obtained in three ways :
- shoot buds developed from meristems located at brackets present on the peduncles of third-order-flowers
- somatic embryos formed on the peduncle of an explanted single young flower-bud
- adventitious buds initiated on explanted flower-buds cut into segments.
A simple medium with growth regulators such as : IAA/1 mg.1-1, GA /0.1 mg.1-1/ and BA /0.8 mg.1-1/ was needed for stimulation of plant regeneration from axillary meristem on the peduncle. For somatic embryos formation and adventitious buds intitiation the medium of Lee and de Fossard (1977) which is rich in a wide range of vitamins, auxins and cytokinins was necessary.
Cultivars differed regarding plantlets formation from sectioned flower-buds. The most successful cultivar was Redgauntlet, little less Surprise des Halles, and much difficult was Senga Sengana.
After transfering the plantlets to a medium containing IBA (0.5 mg.1-1) as the only growth regulator, the process of rooting was accelerated.