MESOPHYLL PROTOPLASTS OF CICHORIUM INTYBUS L. : AN ALTERNATIVE TO MULTIPLICATION AND SELECTION
Our results show that the whole development cycle of Cichorium intybus L. var. Witloof (cv. 'Zoom') can be obtained with protoplasts released from aseptic plantlets. The regeneration rate is about 0.1 to 1 % according to experimental conditions.
An yield improvement in our laboratory will be the result of new investigations on initial density of protoplast, osmotic pressure, composition of culture media and the utility of agar adjunction at the first step of culture.