A NEW PATENTED METHOD FOR MASS PROPAGATION OF SHOOT CULTURES
A new method for propagation of shoot cultures has been invented and worked out experimentally. The main point of the method is that first we establish a shoot culture as usual planting shoot cuttings into solid nutrient medium in a suitable flask, then fill up the cultures with liquid nutrient medium. Both the solid and the liquid media were generally made by Murashige and Skoog /1962 /, but other compositions of salts were suitable too. Usually we put growth regulators, mainly cytokinins and auxins in solid medium, but the liquid medium generally contained only cytokinins. We grow the cultures stationary without any external mechanical aeration on illuminated shelves. It was found that if the shoot cultures were cultivated in the abovementioned circumstances they did not suffer damage, but formed more branches and more axillary buds and shoots. Micropropagation by this method resulted sometimes 2–3 times more cuttings than the usual method. The cuttings were also more vigorous and formed more and stronger roots earlier than by the usual method. Repeating this treatment many times caused no damage to the cultures or to the plants developed from them. All the investigated plant species could grew under above-mentioned circumstances using their optimum media. Up to the present we have used this method with success with many species. These species belong to the Monocotyledoneae, Dicotyledoneae and Filicopsida classes.
Molnár, Gy. (1987). A NEW PATENTED METHOD FOR MASS PROPAGATION OF SHOOT CULTURES. Acta Hortic. 212, 125-130