FACTORS INFLUENCING VITRIFICATION OF CARNATION AND CONIFERS

I. Dencso
This paper deals with some experiences of carnation and conifer propagation carried out by meristem and embryo culture methods. On the base of the experiences it has been stated that Earle-Langhans medium with some modifications is suitable for carnation propagation in vitro. The vitrification was affected by microelement content of the nutrient medium and by the ratio of KIN: NAA the inoculation period, the variety and the properties of mother plant. It was important to reduce microelement level to one-tenth of the amount of MS micro.

A similar result was obtained when we made analyses of macro and microelements in the plants using atomic absorption spectrophotometer. The results showed that if we applied different amounts of microelements from the disposable nutrients the smaller part was taken from the macroelements and the greater part from the microelements. The vitrification rate was reduced on medium containing MS macro with Heller microelements, simultaneously the multiplication rate increased. In this experiment the test plant was gerbera. Vitrification of shoots frequently occured during embryo culture of some conifers. Applying high cytokinin level or activated charcoal increased vitrification, it was also affected by different choice of culture media.

Dencso, I. (1987). FACTORS INFLUENCING VITRIFICATION OF CARNATION AND CONIFERS. Acta Hortic. 212, 167-176
DOI: 10.17660/ActaHortic.1987.212.27
https://doi.org/10.17660/ActaHortic.1987.212.27

Acta Horticulturae