THE MICROPROPAGATION OF PROSOPIS JULIFLORA (SWARTZ) D C: ESTABLISHMENT IN VITRO
Prosopis juliflora (Swartz) D C nodal explants were successfully initiated into aseptic culture with a two stage sterilization procedure. This involved a 2 minute immersion in 70% ethanol and then a 20 minute treatment with sodium hypochlorite (2% available chlorine) and wetter. Kinetin or benzyl adenine were necessary in the medium for shoot extension of axillary buds. Auxins, either indole acetic acid or napthalene acetic acid were not required in the initiation medium, as their inclusion caused callus growth at the base of nodal explants and a reduction in shoot growth. The condition of the mother plant was also shown to influence in vitro performance of the explants.
Wainwright, H. and England, N. (1987). THE MICROPROPAGATION OF PROSOPIS JULIFLORA (SWARTZ) D C: ESTABLISHMENT IN VITRO. Acta Hortic. 212, 49-53