PHOTOSYNTHETIC ACTIVITY OF CAULIFLOWER MERISTEM CULTURES IN VITRO AND AT TRANSPLANTING INTO SOIL
The photosynthetic apparatus of regenerating cauliflower meristem cultures is not sufficiently active to produce a net positive carbon balance in vitro. The chloroplasts have light-stimulated electron transport comparable to control material but lower levels of chlorophyll and ribulose bisphosphate carboxylase activity, resulting in correspondingly low carbon assimilation. This photosynthetic system does not develop further after transplanting as the in vitro foliage deteriorates rapidly, contributing little to net carbon uptake. The problems of transplanting would be eased if the in vitro foliage were persistent and an increase in chlorophyll and RubPcase activity brought about, perhaps by hormonal or environmental manipulation. Such a pattern of development has been observed in a limited number of regenerating cultures and one such, Dieffenbachia, is considered.
Grout, B. W.W. and Donkin, Maria E. (1987). PHOTOSYNTHETIC ACTIVITY OF CAULIFLOWER MERISTEM CULTURES IN VITRO AND AT TRANSPLANTING INTO SOIL. Acta Hortic. 212, 323-328