OPTIMALISATION OF THE TRANSFER OF TISSUE CULTURED SHOOTS TO IN VIVO CONDITIONS

L.J. Maene, P.C. Debergh
Different culture media ingredients and incubation conditions are investigated to optimize uniform shoot elongation at the end of the in vitro phase (StIIIa) and root formation in vivo afterwards (StIIIb). The cytokinin type and concentration and the light intensity in StIIIa are very important.

A production scheme is presented where the in vitro manipulations in the final stage are limited to only one inoculation of plant material on a fresh solid multiplication medium. Uniform elongation and eventually root induction are realised by the addition to the established cultures of an appropriate liquid medium.

Vitrification or similar physiological aberrations can be overcome by the incubation of StIIIa-cultures under high illumination and/or under low relative humidity in the container. This can be practicaly achieved without negative influences on the subsequent rooting in StIIIb by the use of bottom cooling.

Maene, L.J. and Debergh, P.C. (1987). OPTIMALISATION OF THE TRANSFER OF TISSUE CULTURED SHOOTS TO IN VIVO CONDITIONS. Acta Hortic. 212, 335-348
DOI: 10.17660/ActaHortic.1987.212.51
https://doi.org/10.17660/ActaHortic.1987.212.51

Acta Horticulturae