OPTIMALISATION OF THE TRANSFER OF TISSUE CULTURED SHOOTS TO IN VIVO CONDITIONS
A production scheme is presented where the in vitro manipulations in the final stage are limited to only one inoculation of plant material on a fresh solid multiplication medium. Uniform elongation and eventually root induction are realised by the addition to the established cultures of an appropriate liquid medium.
Vitrification or similar physiological aberrations can be overcome by the incubation of StIIIa-cultures under high illumination and/or under low relative humidity in the container. This can be practicaly achieved without negative influences on the subsequent rooting in StIIIb by the use of bottom cooling.