IN VITRO PROBLEMS RELATED TO PROPAGATION OF DIFFERENT FERN SPECIES
Apogamous sporophyte organisation has been observed in the species Pteridium aquilinum, Cyclosorus contiguus, Dryopteris filixmas, Pteris henryi, Osmunda regalis and recently in Pteris cretica cv. albolineata. Cytological test proved their haploid level.
Sporophytes of Marsilea quadrifolia and Pilularia globulifera could be also regenerated from excised shoots.
Four different callus types were observed and investigated.
Sporophytes are maintained on half-strength (MS/2) hormon free media and then transferred to soil.