EXPERIENCES ON THE 'IN VITRO' PROPAGATION OF SOME CITRUS ROOTSTOCKS

A. Starrantino, A. Caruso
The citrus rootstocks 'Troyer' and 'Carrizo' citranges (Citrus sinensis L. Osbeck x Poncirus trifoliata Raf.), 'Flying Dragon', trifoliate orange (P. trifoliata) 'and a 'dwarf mutant' seedling of 'Troyer' citrange have been successfully 'in vitro' propagated since 1982 using shoot apices (0.5 – 1 cm. long).

The best results have been obtained when the shoots of the 'Troyer' and 'Carrizo' citranges were 'in vitro' cultured on basal medium(MS) supplemented with BA 1 mg/l., IBA 0.5 mg/l. and adenine 40 mg/l., while the 'Flying Dragon' and the 'dwarf mutant' showed the highest multiplied rate with BA 0.5 mg/l., IBA 0.25 mg/l. and adenine 40 mg/l.

The root emission has been established on the same basal medium (MS) supplemented with NAA 1 mg/l.

After 45 days from the root initiation the seedlings have been transplanted in sterilized soil and peat mixture. Thus many seedlings have been obtained.

After three years of subculturing the shoot proliferations showed a constant multiplication and rooting capacity.

Furthermore no morphological mutation has been observed in the trees obtained and the chromosome number in the root-tips resulted normal (2X = 18).

Starrantino, A. and Caruso, A. (1987). EXPERIENCES ON THE 'IN VITRO' PROPAGATION OF SOME CITRUS ROOTSTOCKS. Acta Hortic. 212, 471-478
DOI: 10.17660/ActaHortic.1987.212.72
https://doi.org/10.17660/ActaHortic.1987.212.72

Acta Horticulturae