IN VITRO ESTABLISHMENT AND MULTIPLICATION OF JUVENILE AND MATURE DOUGLAS-FIR AND SUGAR PINE

P.K. Gupta, D.J. Durzan
With cotyledons of 2 to 3-week-old seedlings of Douglas-fir (DF) and sugar pine (SP), numerous adventitious buds were induced by a newly formulated basal medium (DCR) supplemented with N6-benzyl-aminopurine (BAP) and alpha-naphthalene acetic acid (NAA). Buds were elongated on 0.5 DCR alone. Apical and axillary buds of mature trees were flushed on DCR without growth regulators. Buds were elongated on 0.5 DCR with 3% (w/v) activated charcoal (AC). Shoots were multiplied on subculture on DCR supplemented with 0.2–0.5 mg/L BAP. Rooting of micropropagated shoots of DF from both juvenile and mature trees treated by these methods is now easier with NAA. Plantlets have been successfully transferred to the field.
Gupta, P.K. and Durzan, D.J. (1987). IN VITRO ESTABLISHMENT AND MULTIPLICATION OF JUVENILE AND MATURE DOUGLAS-FIR AND SUGAR PINE. Acta Hortic. 212, 483-488
DOI: 10.17660/ActaHortic.1987.212.74
https://doi.org/10.17660/ActaHortic.1987.212.74

Acta Horticulturae