IN VITRO PROPAGATION OF ADULT MATERIAL AND SEEDLINGS OF CORYLUS AVELLANA
Adult and seedling explants of Corylus avellana L. cv. Gironell were cultured in vitro on different cytokinins supplemented media. The cytokinins tested were Zeatin, BA, 2 i P and Kinetin at concentrations ranging from 0 to 20 mg/l. The basal medium was the MS formulation at half strengh supplemented with 0, 01 mg/l AIA, 2 mg/l GA, 30000 mg/l saccharose and 8000 mg/l agar. Shoot segments about 2–3 cm long with one vegetative bud were used as explants. Cytokinins tested promote hazelnut budding at dose between 2,5 – 10 mg/l. Adult explants had given less multiplication rate that juvenile ones at all concentration tested but with similar behaviour patterns. This suggest that the micropropagation is not only a problem concerning with chemical composition of culture media but that hazelnut presents juvenility-agening problems similar to other woody plant species, and it would be necessary to submit adult material to a rejuvenation process simultaneusly to optimizise culture medium.
Messeguer, J. and Melé, E. (1987). IN VITRO PROPAGATION OF ADULT MATERIAL AND SEEDLINGS OF CORYLUS AVELLANA. Acta Hortic. 212, 499-504