EXPERIENCES AND PROBLEMS RELATED TO THE MICROPROPAGATION OF BLACK LOCUST
Shoot tips of four new varieties were used as explants to start culture. After desinfection with 0,5 p.c. HgCl2 for 1 min. multiple shoot formation was induced on modified MS-medium supplemented with various concentrations of BAP (0,1 – 2,0 mgl-1) and adenine - sulfate (1–2 mgl-1).
Multiplied shoots were subcultured to root to a low salt medium containing different concentrations of auxins. These rooted plantlets were transferred to pot in a green-house.
Although plantlets could be potted and they are growing in the field the rate of rooting hasn't been satisfactory till now and has to be raised.