IN VITRO PLANTLET REGENERATION FROM JUVENILE AND MATURE SYCAMORE MAPLE
The juvenile explants were seedlings cultured at the 2–4 node stage. Initial cultures of mature trees (60 to 100 y) were established using herbaceous sprouts collected during summer from stumps a few months to several years after tree felling.
The most satisfactory medium included Murashige and Skoog's mineral formula, organic components listed by Bourgin and Nitsch, both diluted three times. Fe-EDTA, sucrose, agar and activated charcoal were added. The adding of growth regulators was not required to induce rhizogenesis.
Regenerated plantlets were successfully transfered into soil.