ORGANOGENESIS IN HYBRID COCONUT EMBRYO CULTURE
In vitro multiplication of coconut palms was attempted using embryo culture. Embryos from mature nuts of Bali x Nias hybrid (KINA II) were initially cultured in a half-strength Eeuweens Y3 liquid medium containing 0.1 mg/1 NAA and 2% sucrose. After 2 weeks in the liquid phase, the embryos were sliced and transferred to a full-strength Y3 agar medium containing 0.2, 1, 5 and 10 mg/1 2, 4-D, 0.25% activated charcoal and 3% sucrose. Embryos initially conditioned in the liquid medium underwent extensive tissue growth. Subsequent culture of the cotyledonary slices from the enlarged embryo on agar medium containing 5 or 10 mg/1 2,4-D stimulated nodular proliferations resembling protocorms of orchid callus. These nodules could be subcultured with reduced level of 2,4-D, eventually reverting to callus, or in combination with BAP to stimulate further nodular development suggestive of shoot organization. These nodules developed roots in the presence of NAA.
Tahardi, S. (1987). ORGANOGENESIS IN HYBRID COCONUT EMBRYO CULTURE. Acta Hortic. 212, 567-569