TISSUE CULTURE PROPAGATION OF PAPAYA
We report here the procedure for tissue culture propagation of a dioecious variety.
The explant were axillary buds of three years old field plants. Sterilization was carried out by Rifampicin and NaOC1 solution. Initiation media was modified MS. Multiplication media was MS supplemented with 0.5 ppm BA and 0.1 ppm NAA. Rooting was carried out by immersing the explants in 1 ppm IBA for 12–18 hours and then transfer to liquid MS basal medium. Internal bacterial contamination was a serious obstacle. The question of somaclonal variation and the cost of the procedure is yet to be studied.