EFFECT OF CO2 ENRICHMENT AND HIGH PHOTOSYNTHETIC PHOTON FLUX ON THE DEVELOPMENT OF AUTOTROPHY AND GROWTH OF TISSUE-CULTURED STRAWBERRY, RASPBERRY AND ASPARAGUS PLANTS.
The research objectives were to determine the effects of high photosynthetic photon fluxes (PPF) and CO2 enrichment on the growth and anatomy of tissue-cultured plants and to determine what is the contribution of these factors in the development of autotrophy in vitro. Nine specially designed thermo-regulated growth chambers have been assembled to study these factors without interaction from temperature or humidity. These low cost chambers maintained a uniform temperature within 1 °C of a set point. Three levels of CO2 (600, 1500 and 3000 vpm) and three PPF (80, 150 and 250 μmol.m-2.s-1) were studied with raspberry, strawberry and asparagus. In vitro plants had a significant photosynthetic carbon metabolism as was observed from the important decrease in the test tube CO2 concentration, at the onset of photoperiod. Plants raised under low CO2 environment reached levels of CO2 in the test tube below 100 vpm. High CO2 and PPF increased the fresh weight of asparagus plantlets. For strawbery, 14CO2 fixation was slightly improved by high CO2 atmosphere in the presence of sucrose in the medium. High CO2 and PPF were shown to influence the anatomy of raspberry plantlets by increasing the stomatal density. It is not yet known however if the plantlets have a better performance in the acclimatization stage.
Desjardins, Y., Laforge, F., Lussier, C. and Gosselin, A. (1988). EFFECT OF CO2 ENRICHMENT AND HIGH PHOTOSYNTHETIC PHOTON FLUX ON THE DEVELOPMENT OF AUTOTROPHY AND GROWTH OF TISSUE-CULTURED STRAWBERRY, RASPBERRY AND ASPARAGUS PLANTS.. Acta Hortic. 230, 45-54