STUDIES ON NUCLEAR PROTEINS OF TULIP: NUCLEAR ISOLATION AND PROTEIN FRACTIONATION

W.F. Sheridan
Tulip leaves of cv. 'Golden Age' were disrupted by blending in dry ice. Nuclei were isolated after dehydration in cold acetone by centrifugation in carbon tetrachloride-cyclohexane mixtures of varying densities. The purest nuclear fraction was obtained at a density between 1. 450 and 1. 470 at 0°C. DNA assays indicate about a five fold purification of nuclei Soluble proteins, histones, chromosome residual proteins, and nuclear residual proteins were prepared and analyzed for protein and DNA content.
Sheridan, W.F. (1971). STUDIES ON NUCLEAR PROTEINS OF TULIP: NUCLEAR ISOLATION AND PROTEIN FRACTIONATION. Acta Hortic. 23, 142-149
DOI: 10.17660/ActaHortic.1971.23.22
https://doi.org/10.17660/ActaHortic.1971.23.22

Acta Horticulturae