EFFECTS OF TEMPERATURE, LIGHT, SUGARS AND GIBBERELLIN A3 ON THE GROWTH IN VITRO OF TERMINAL BUDS OF TULIP
The growth of terminal buds of tulips (holding a complete flower) isolated from bulbs of the cv. 'Apeldoorn' was investigated. The bulbs were pre-treated by dry-storage at 5°C for 0, 6, or 12 weeks. The buds were grown on a basic medium containing sugars and gibberellin A3 (GA3). The growth of isolated buds was promoted by increasing the duration of the 5°C pre-treatment and retarded by cultivation at low temperature, and the presence in the medium of fructose, glucose or sucrose. Darkness gave a slight retardation of growth. Growth was promoted by GA3. In most cases growth promotion was accompanied by early flower blasting. Flowering was obtained with explants from bulbs treated at 5°C for 6 weeks and cultivated in light, first at 9°C and then at 18°C, on a medium containing sucrose and GA3.
van Bragt, J. (1971). EFFECTS OF TEMPERATURE, LIGHT, SUGARS AND GIBBERELLIN A3 ON THE GROWTH IN VITRO OF TERMINAL BUDS OF TULIP. Acta Hortic. 23, 429-440