EFFECT OF ERICOID MYCORRHIZAE ISOLATES ON GROWTH AND DEVELOPMENT OF LOWBUSH BLUEBERRY TISSUE CULTURE PLANTLETS*
A randomized complete block design with 36 blocks and 8 treatments used tissue culture plantlets of clone NB-3 which had been rooted in a peat/perlite/vermiculite medium and grown in Can-Am containers. Treatments were 3 fungal isolates—two local and Hymenoscyphus ericae from England—and uninoculated control, all with and without fertilizer. Inoculant was macerated mycelium scraped from plates of modified Melin-Norkrans medium. Fertilizer was 18–6–12 Osmocote, 0.2 g on surface. Height and branching were noted after 10 weeks of greenhouse growth and dry weight measured after 22 weeks. Total nitrogen content of aerial plant tissue was determined. Infection was rated on three root samples from 3 randomly selected blocks, each of 8 treatments in well-separated compartments. Fertilizer increased height, branching, dry weight and total nitrogen content of controls and inoculated plantlets. Controls showed infection, presumably from propagules in the peat. Infection levels were significantly less in the fertilized plantlets than in the unfertilized. In similar experiments with clones ME-3, 723, and Chignecto, fertilizer did not suppress colonization, but results for growth and nitrogen content were essentially the same as in clone NB-3.
Smagula, John M. and Litten, W. (1989). EFFECT OF ERICOID MYCORRHIZAE ISOLATES ON GROWTH AND DEVELOPMENT OF LOWBUSH BLUEBERRY TISSUE CULTURE PLANTLETS*. Acta Hortic. 241, 110-114