COMPARISON BETWEEN 2iP AND ZEATIN IN THE MICROPROPAGATION OF HIGHBUSH BLUEBERRY (VACCINIUM CORYMBOSUM)
The effect of zeatin was compared with that of 2iP in establishment and proliferation phases. Rooting and acclimatation of micropropagated plants were also studied.
Zeatin proved to be less phytotoxic than 2iP, especially at higher doses. During establishment a greater number of sprouted explants was obtained with zeatin than with 2iP; therefore zeatin could advantageously substitute 2iP, at least in this phase.
Number and length of shoots in proliferation phase was mostly dependent on the total level of cytokinins used, but the best general appearance of cultures was obtained with both zeatin and 2iP in the medium.
No problems were found in rooting propagules in vitro, while their acclimatation under wet tent was influenced by the composition of the substratum.