NAA EFFECT ON PHENOLASE ACTIVITY IN RUSCUS RACEMOSUS MOENCH DURING IN VITRO PROPAGATION.
Phenolase activity has been studied in Ruscus racemosus Moench explants cultivated in vitro. The enzymatic activity was found to increase in the explants after addition, onto the jelled culture medium surface, of a solution containing NAA 2 mg/l. The enzyme, extracted from fresh material, has been partially purified using DEAE Cellulose column chromatography, and its activity has been tested with 4-methyl catechol buffered solution containing Ascorbic Acid and EDTA, recording the Absorbance decrease at 265 nm wawelength. A part of phenolic compounds present in the explants were identified, and among these (+) catechin resulted able to act as substrate for the enzyme. The hypothesis of a link between enzymatic activity increase in the explants and their in vitro rhizogenetic potentialities expression is discussed.
Curir, P., Termini, A., Ruffoni, B. and Marchesini, A. (1989). NAA EFFECT ON PHENOLASE ACTIVITY IN RUSCUS RACEMOSUS MOENCH DURING IN VITRO PROPAGATION.. Acta Hortic. 251, 135-140