SHOOTS MULTIPLICATION AND ROOTING OF HOSTA SIEBOLDIANA CV. GOLD STANDARD USING CULTURED SHOOT TIPS

Shoot tips proliferated in vitro were used as explants to determine the effects of various combinations and concentrations of growth substances /KIN, 2iP, BAP with IAA,NAA,IBA/ on the multiplication and rooting of shoots. Three kinds of media were investigated - agar medium, liquid shaking medium and liquid medium with filter paper bridge.

The highest shoot multiplication /5.3 shoots par explant/ was obtained on liquid medium with filter paper bridge /NAA 0.6 mg/l and BAP 4 mg/l/, but the shoots showed vitrification symptoms. The shoots without vitrification were obtained on agar medium /4.6 shoots per explant/ containing NAA 0.1 mg/l and BAP 6 mg/l. The rooting of shoots was effective on solid medium without growth substances but was better with the use of NAA 0. 1 mg/l.