REGENERATION OF TWO CULTIVARS OF ACHIMENES LONGIFLORA DC. IN VITRO
Shoot and root regeneration was optimum when leaf explants were used in comparison to stem or pedicel segments; supplementing the basic medium with 10-7 g/ml BA and 5.10-7 g/ml NAA in cv Flamenco and with 5.10-7 g/ml BA and 10-6 g/ml NAA in cv English Waltz the highest number of shoots was produced. Along with shoots, rhizomes were also formed.
In 'Flamenco' fresh weight of shoots and rhizomes was enhanced when explants were taken from the older leaves of stock plants grown at 17° rather than at 25 °C and a light regime of a basic 12 h illumination period extended for 6 h with low intensity incandescent lamps (GLS) rather than with compact gas-discharge lamps (SL*). Regeneration of 'English Waltz' was not affected by temperature pretreatment of stock plants. The highest number of rhizomes in 'English Waltz' was recorded when BA was combined with NAA at 5.10-7 g/ml each.