STRATEGIES FOR SEED BACTERIZATION
In this study, emphasis is on seed rhizobacterization with Pseudomonas fluorescens and P. putida and specially on two main difficultes :
- How to do a judicious selection of PGPR strains able to produce a well-adapted inoculum ?
- How to perform an effective delivery system of bacteria applied to the seed allowing a fast establishment of inoculum on the radicle during germination ?
Moreover effectiveness of bacterial seed coating is spatial-limited by seed bulk itself and by development of root system causing a gradual dilution of bacterial inoculum. So seed rhizobacterization can be complemented by soil bacterization in the root zone, performed simultaneously when sowing. In the future, genetic engineering could improve the effectiveness of seed rhizobacterization by establishing artificial symbiosis between bacteria and root system.