ETHYLENE-REGULATED GENE EXPRESSION DURING CARNATION PETAL SENESCENCE

W.R. Woodson, K.A. Lawton, P.B. Goldsbrough
Senescence of carnation flower petals is a highly regulated developmental process which requires active gene expression and protein synthesis. Isolation and in vitro translation of petal mRNAs revealed that petal senescence is associated with the expression of specific mRNAs, several of which appear concomitant with the ethylene climacteric. In order to characterize the mRNAs expressed during petal senescence and determine the role of ethylene in their expression, a cDNA library was constructed in the phage cloning vector lambdagt10 using mRNA from senescing petals as the cDNA template. Cloned cDNAs that correspond to mRNAs expressed specifically during petal senescence were isolated by differential hybridization. These cDNAs hybridize to distinct mRNAs based on northern analysis. The cDNAs represent two classes of mRNAs. One class represents mRNAs whose expression is highly regulated by ethylene. These mRNAs are not present in significant amounts before the ethylene climacteric associated with petal senescence, but accumulate rapidly during the period of autocatalytic ethylene synthesis. This group of mRNAs can be induced by ethylene in young petals and is not expressed in aging petals pretreated with inhibitors of ethylene biosynthesis or action. The second class of cDNAs corresponds to mRNAs that increase in abundance during the course of petal maturation and prior to the ethylene climacteric. Ethylene can increase their abundance in young petals, but treatment with inhibitors of ethylene biosynthesis or action does not completely prevent their accumulation in aging petals. Thus, the expression of these genes is more temporally regulated and less responsive to ethylene.
Woodson, W.R., Lawton, K.A. and Goldsbrough, P.B. (1989). ETHYLENE-REGULATED GENE EXPRESSION DURING CARNATION PETAL SENESCENCE. Acta Hortic. 261, 137-144
DOI: 10.17660/ActaHortic.1989.261.17
https://doi.org/10.17660/ActaHortic.1989.261.17

Acta Horticulturae