THE USE OF MERISTEM TIP CULTURE TO ELIMINATE VIRUSES FROM STRAWBERRY PLANTS
We tested a technique for virus elimination by meristem tip culture. With this method we have succeeded in producing healthy plants. We took very small pieces (0,2 – 0,3 mm) of apical meristem and we planted them in M.S. nutrient medium (Muraschige and Skoog, 1962) suplemented with organic constituents of glucose and agar (Boxus, 1974). It was a basic medium. On this medium suplemented with 1 mg/1 IBA, O,1 mg/1 6-BAP we succeeded in getting sprouts in 40–50% of cases. The sprouts were aseptically transfered into a basic medium with the adition of IBA 1 mg/1 and 6 - BAP 1 mg/l. After 4–6 weeks of incubation in a climate chamber, the sprouts were multiplied by forming axillary buds so there were 10–15 new sprouts. The sprouts from axillary buds were transfered into basic medium with the addition of 1 mg/1 IBA where they produced roots and leaves. After next 4–6 weeks 95% of thee plants formed roots. Those plants were tested for viruses. It was confirmed that no economicaly major virus diseases were present.
Kajic, V. and Cvjetkovic, B. (1989). THE USE OF MERISTEM TIP CULTURE TO ELIMINATE VIRUSES FROM STRAWBERRY PLANTS. Acta Hortic. 265, 595-598