HISTOCHEMICAL TEST FOR PRESENCE OR ABSENCE OF CHALCONES IN ANTHERS FROM BULBS OF TULIP CV. APELDOORN PRECOOLED AT 5 °C OR KEPT AT 17 °C

N. Gorin, R. Sütfeld, J. Tonecki, J.H. Franssen, N. Haanappel
Chalcones in anthers from tulip bulbs were detected by suspending 1–3 anthers in several drops of 4–5 M KOH (total volume 0.4 ml). The orange-red pigmentation in the anthers appearing within 10–60 min (positive reaction) denoted the presence of chalcones (2',3,4,4',6'-pentahydroxychalcone, 2',4,4',6'-tetrahydroxychalcone, 2',4,4,6'-tetrahydroxy-3-methoxychalcone). The minimum amount of chalcone detected by the test was 320 nmol, as determined with synthetic 2',4,4',6'-tetrahydroxychalcone.

Using this simple histochemical test, we found that chalcones were not present in anthers from bulbs stored at 5 °C for 12 weeks, if storage began before September 22. However, when storage began on October 3rd-5th, chalcones were detected in 4–6 weeks. Moreover, chalcones were always detected in anthers from bulbs cooled at 5 °C for 12 weeks and subsequently kept at 17 °C for one week (transport simulation). Chalcones were also first detected in anthers from bulbs kept at 17 °C (controls) after 2–8 weeks, depending on the date storage began. The earlier storage began, the later was the positive reaction.

A negative result could be used to indicate uninterrupted cooling but only for special precooled bulbs cv. Apeldoorn for which cold treatment ended during the first two weeks of December.

Gorin, N., Sütfeld, R., Tonecki, J., Franssen, J.H. and Haanappel, N. (1990). HISTOCHEMICAL TEST FOR PRESENCE OR ABSENCE OF CHALCONES IN ANTHERS FROM BULBS OF TULIP CV. APELDOORN PRECOOLED AT 5 °C OR KEPT AT 17 °C. Acta Hortic. 266, 221-228
DOI: 10.17660/ActaHortic.1990.266.28
https://doi.org/10.17660/ActaHortic.1990.266.28

Acta Horticulturae