A.M.G. Paffen, P. Aguettaz, I. Delvallée, G.J. de Klerk, R.J. Bogers
We have studied the effects of culture conditions on dormancy of bulblets generated in vitro on scale explants of Lilium speciosum Thunb. cv. 'Rubrum' nr. 10. Bulblets generated under standard conditions (normal strength of Murashige and Skoog salts, 3% sucrose, 11 weeks of culture at 20°C) were dormant upon harvest and needed a cold treatment to break dormancy. At low temperature (15°C), high GA3 concentration (1 mg.l-1) or low sucrose concentration (10 g.l-1 or less) only a low level of dormancy developed during 11 weeks of culture. Daylength, light or dark, or application of various concentrations of abscisic acid, 6-benzylaminopurine, 1-naphthaleneacetic acid, indole-3-acetic acid, 2,3,5-triiodobenzoic acid or Murashige and Skoog salts had no effect. No correlation was found between morphological characteristics and the dormancy status of the bulblets.

Dormancy developed after 8 weeks of culture under standard conditions. At high temperature (25°C) dormancy developed earlier, but at low temperature (15°C) hardly any dormancy was induced, even during protracted culture (up to 26 weeks). Low sucrose concentration (10 g.l-1 or less) delayed the development of dormancy, but had no effect on its final level.

Paffen, A.M.G., Aguettaz, P., Delvallée, I., de Klerk, G.J. and Bogers, R.J. (1990). THE DEVELOPMENT OF DORMANCY IN LILY BULBLETS GENERATED IN VITRO. Acta Hortic. 266, 51-58
DOI: 10.17660/ActaHortic.1990.266.5

Acta Horticulturae