IN VITRO BULBLET PROPAGATION OF VIRUS-FREE DUTCH IRIS.
In vitro bulblet development of Dutch iris is optimized with the following media constituents and concentrations: 6% sucrose, full strength Murashige Skoog inorganics, 1231 uM NaH2PO4H2O, 555 uM myo inositol, 1.2 uM thiamine HCl, pH adjusted to 5.7, and 0.6–0.8% agar. Once shoot multiplication and intermediate stages had been completed the shoots were transferred to the bulblet development medium. The cultures were cooled 5° C for 4 weeks and then transferred to 30°C for 5–8 weeks. The duration of heat period was limited by shoot sprouting; as the treatment was terminated when sprouting began. Immediately following the 30°C period, the bulblets were again cooled at 5°C for 4 weeks before planting in the greenhouse. Bulblets required 3 greenhouse cycles to develop sufficient bulb size for daughter bulb multiplication.
Anderson, Wilbur C., Mielke, K. A., Miller, P. N. and Allen, T. (1990). IN VITRO BULBLET PROPAGATION OF VIRUS-FREE DUTCH IRIS.. Acta Hortic. 266, 77-82