IN VITRO REGENERATION OF PLANTLETS OF CANARY ISLAND PINE

C. Martínez Pulido, I.S. Harry, T.A. Thorpe
Adventitious buds of Canary Island pine (Pinus canariensis) were induced on 3-day-old cotyledonary explants cultured on Bornman's MCM medium supplemented with cytokinin. Bud development was enhanced by the elimination of phytohormones, a reduction of mineral salts and sucrose, and the inclusion of activated charcoal (AC) in the medium. Shoots were pulsed in a high concentration of indole butyric acid (IBA) and rooted in peat/vermiculite.
Martínez Pulido, C., Harry, I.S. and Thorpe, T.A. (1991). IN VITRO REGENERATION OF PLANTLETS OF CANARY ISLAND PINE. Acta Hortic. 289, 131-132
DOI: 10.17660/ActaHortic.1991.289.29
https://doi.org/10.17660/ActaHortic.1991.289.29

Acta Horticulturae