RESEARCHES ON OBTAINING DIPLOIDE PLANTS IN VITRO FROM THE PISTILS OF NICOTIANA RUSTICA L.

D. Basaran, G. Çolak, O. Namli, A. Onay, S. Yücel
For these researches, we took the boutons of N.rustica L. ten day before flowering. We sterilized them in ethyl alcohol 70 % during 30 seconds and in sodium hypochlorite of 3 % during 35 minutes. We rinsed them with distiled water and sterilized 4 times during 5 minutes.

Later, we took out only the pistils and put them in Murashige and Skoog medium which contain two sorts of composition of hormone: first solution contains 4 mg/1 of kinetine and 1 mg/1 IAA; the second contains 4 mg/1 of K and 1 mg/1 naphtylacetic acid.

Finally, we obtainede diploide plants only from the stumps of IAA media.

Basaran, D., Çolak, G., Namli, O., Onay, A. and Yücel, S. (1991). RESEARCHES ON OBTAINING DIPLOIDE PLANTS IN VITRO FROM THE PISTILS OF NICOTIANA RUSTICA L.. Acta Hortic. 289, 215-216
DOI: 10.17660/ActaHortic.1991.289.50
https://doi.org/10.17660/ActaHortic.1991.289.50

Acta Horticulturae