FURTHER STUDIES ON THE PHOTOSYNTHETIC COMPETENCE AND ACCLIMATIZATION OF ROSA SPECIES EXPLANTS
Plantlets of Rosa hybrida cvs. Mountbatten and City of Leeds were proliferated on agar based Murashige and Skoog (MS) media containing Sucrose (3 and 4% respectively), BAP (2mg/l), NAA (0.04mg/l, and 0.004mg/l, respectively) and GA3 (0.1mg/l), for 4–6 weeks. Explants were transferred to MS media supplemented with a range of sucrose concentrations (0–5%) and to a simple (sugar-free) hydroponic nutrient medium. Growth rates were recorded and measurements of the carbon dioxide assimilation rates made using an Infra-Red Gas Analyser (IRGA). The use of culture vessels which permitted increased levels of gaseous exchange, together with a reduction in humidity, facilitated the development of photosynthetic competence within explants grown on MS medium supplemented with sucrose and on the simple hydroponic medium. The use of a hydroponic nutrient solution in combination with transplantation plugs for both rooting and acclimatization of explants is also discussed.
WALKER, S., SIMPKINS, I. and ROBERTS, A.V. (1991). FURTHER STUDIES ON THE PHOTOSYNTHETIC COMPETENCE AND ACCLIMATIZATION OF ROSA SPECIES EXPLANTS. Acta Hortic. 289, 347-348